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KMID : 1134820100390050692
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Journal of the Korean Society of Food Science and Nutrition
2010 Volume.39 No. 5 p.692 ~ p.698
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Anti-diabetic Activities of Kocat-D1 in 3T3-L1 Adipocytes and C57BL/KsJ-db/db Mice
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Yang Ji-Hee
Won Hye-Jin
Park Ho-Young
Nam Mi-Hyun
Lee Hyun-Sun
Lee Joong-Ku
Kim Jong-Tak
Lee Kwang-Won
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Abstract
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This study investigated anti-diabetic activity of Kocat-D1, which is a currently used traditional medicine for treatment of diabetes in Shandong, China. Insulin sensitizing activity was observed in a cell-based glucose uptake assay using 3T3-L1 adipocytes. The treatment of 0.2 ㎎/㎖ of hot water extract of Kocat-D1 with 0.2 nM insulin was associated with a significant increasing in glucose uptake (165.0±0.7%) over the treatment of 0.2 nM insulin. C57BL/KsJ-db/db mice (8 weeks of age) were separated into 3 groups: normal control (control, db/＋ mice untreated), diabetic control (DM control, db/db mice untreated), Kocat-D1 (db/db mice treated with Kocat-D1 extract 350 ㎎/㎏/day). After 16 weeks of treatment, body weight and total diet intake of Kocat-D1 group were significantly lower than DM control groups. Blood glucose levels of the Kocat-D1 group (14.7±1.4 m㏖/ℓ) were significantly lower compared to the DM control group (27.1±0.2 m㏖/ℓ). Furthermore, insulin level was significantly increased in the Kocat-D1 group (0.17±0.02 ng/㎖) compared with the DM control group (0.05±0.02 ng/㎖). The glomeruli in kidney was stained using periodic acid-shiff base (PAS) for confirming collagen accumulation. The glomeruli in kidney of Kocat-D1 group had significantly reduced PAS-positive compared with that of DM control.
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KEYWORD
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Kocat-D1, anti-diabetes, 3T3-L1, C57BL/KsJ-db/db mice, insulin
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